Northwestern Spain Research Articles

Introduction of the South American slipper limpet Crepipatella dilatata in Europe: Secondary spread in the Northeast Atlantic and first records for the Mediterranean Sea

The slipper limpet Crepipatella dilatata, native to Chile and Argentina, was introduced in north-western Spain in 2005 and since then has spread to the Galician and Cantabrian coasts. In this work, we provide the first molecularly validated record of an established population of C. dilatata in central Portugal (Aveiro), and of reproductive individuals (i.e. brooding females) of C. dilatata in the Mediterranean Sea, from three Italian mussel farms (in eastern Sardinia and northern Adriatic). DNA barcoding and phylogenetic analyses based on mitochondrial markers indicate that limpets from Italian farms belong to the C. dilatata ‘clade1’, whereas limpets collected in Aveiro cluster within the C. dilatata ‘clade2’. All these limpets share the same COI haplotypes with introduced populations from Galicia. The arrival of C. dilatata to Europe is undoubtedly due to transoceanic live mussel trade from southern Chile to Galicia. Mussel trade and farming are also the most likely drivers for the post-border dispersal of C. dilatata from Spain to Portugal and to Italy. Several countries have a potential role as a hub for the primary and secondary dispersal of slipper limpets towards and within Europe. This calls the attention for future research focused on assessing the current occurrence and ecological impact of established populations of slipper limpets within and nearby mussel farms along the eastern Atlantic and Mediterranean coasts.

Language Policy in Galicia, 1980-2020. An Overview


 
 
 
 Galician is a minority language spoken in Galicia, an autonomous region in northwestern Spain. This paper will provide some basic data on the evolution of the sociolinguistic situation of Galician. It will consider the dynamics of change and revitalisation of the language and will examine the linguistic policies that underpin them. In 1981, the Statute of Autonomy of Galicia was approved, establishing the co-official status of Galician. In 1983, the Galician Parliament passed the Law on the linguistic normalisation of Galicia, which laid the ground for the language policies of successive autonomous governments. After four decades, there are several symptoms that the language policy is inadequate for promoting Galician, based on a non-confrontational model centred on the teaching and learning of Galician and the promotion of its literary and cultural prestige.
 
 
 

Presence of gastrointestinal and bronchopulmonary parasites in Cantabrian brown bears

The Cantabrian brown bear (Ursus arctos) population is threatened although in a constant process of recovery during the last 20 years. Since data on the parasitological status of this bear is still limited, the objective of the present study was to assess the diversity and prevalence of parasites in this population. Thus, 111 bear faecal samples were collected in north-western Spain and analysed for estimating the occurrence of gastrointestinal and bronchopulmonary parasites. Samples were processed by flotation in saline and sucrose solution, sedimentation and Baermann-Wetzel techniques. In addition, a commercial immunofluorescent assay was performed for detecting Giardia duodenalis and Cryptosporidium spp. Dicrocoelium dendriticum was the most prevalent parasite (58.6%), followed by Baylisascaris transfuga (43.2%) and nematodes of the Suborder Strongylida (18.9%) and Spirurida (2.7%). Mixed infections were detected in the 41.4% of the samples. The presence of D. dendriticum was significantly highest in bears from the autonomous region of Castile and León as well as in those in which grass or nuts/acorns were the predominant food item. Moreover, the risk of being positive to B. transfuga was significantly higher during autumn–winter, and in those, faecal samples were mainly composed of fleshy fruit. Some of the parasites detected could infect other wildlife and even humans, and therefore, the risk of pathogen transmission deserves further investigation. Since the impact of endoparasites in the health status of bears is poorly understood, the establishment of a disease surveillance protocol is strongly recommended in order to assess the potential risk of these infections for bears.

The Portuguese man-of-war: Adrift in the North Atlantic Ocean

On 11 February 2014, dozens of Portuguese man-of-war specimens were washed ashore along the entire stretch of Patos Beach (northwestern Spain). Here we performed both field experiments and numerical simulations to estimate the possible trajectories and region of origin of this swarm. On the one hand, our field experiments confirm the need to consider the main characteristics of an organism when predicting its drift. On the other hand, the numerical simulations show that this swarm originated in the open North Atlantic Ocean. The drift of the swarm was controlled by the succession of storms that hit the coasts of Western Europe in the winter of 2013–14. Our findings also suggest that most of the individuals of the swarm were right-handed.

New threats in the recovery of large carnivores inhabiting human-modified landscapes: the case of the Cantabrian brown bear (Ursus arctos)

Understanding mortality causes is important for the conservation of endangered species, especially in small and isolated populations inhabiting anthropized landscapes where both natural and human-caused mortality may hinder the conservation of these species. We investigated the mortality causes of 53 free-ranging brown bears (Ursus arctos) found dead between 1998 and 2023 in the Cantabrian Mountains (northwestern Spain), a highly human-modified region where bears are currently recovering after being critically threatened in the last century. We detected natural traumatic injuries in 52.63% and infectious diseases in 39.47% of the 38 bears for which the mortality causes were registered, with 21.05% of these cases presenting signs of both infectious diseases and traumas. More specifically, almost 30% of the bears died during or after intraspecific fights, including sexually selected infanticide (10.53%). In addition, primary infectious diseases such as infectious canine hepatitis, distemper, clostridiosis and colibacillosis caused the death of 15.79% of the bears. The number of direct human-caused deaths (i.e., shooting, poisoning, snare) decreased over the study period. This study also reveals three new mortality causes triggered by pathogens, two of which—Clostridium novyi and verotoxigenic Escherichia coli—not previously described in ursids, and the other one, canine distemper virus, never reported in brown bears as cause of death. New management strategies for the conservation of Cantabrian bears, which are urgently needed due to the rapid expansion of the population, should consider the mortality causes described in this study and must promote further research to elucidate how the high prevalence of infectious diseases may threaten the current recovery of the population.

Identification and Characterization of Relict Olive Varieties (Olea europaea L.) in the Northwest of the Iberian Peninsula

Olives (Olea europaea L.) are an important crop in the Mediterranean Basin, but it is not well-known that they have also been grown in other areas, such as Galicia in northwestern Spain. Although commercial production ended long ago in this peripheral growing region, it remains home to olive resources that are well-adapted to the prevailing environmental conditions, providing a valuable but largely undocumented source of genetic variation. Following a survey of Galicia to locate examples of centuries-old olive trees, those detected were subjected to molecular characterization using a set of microsatellite markers, as well as full botanical characterization using the features established by the International Union for the Protection of New Varieties of Plants, along with others proposed by the present authors. These procedures allowed 11 undescribed varieties to be identified, which are new genetic resources that might be of use in olive improvement programs or studies of how the species adapts to different climates. The trees also underwent preliminary health checks, allowing disease-free specimens of each variety to be propagated. The addition of this material to the Community Plant Variety Office’s register of commercial varieties is underway.

Language revitalization through a social movement lens: grassroots Galician language activism

AbstractIn this article, a social movement lens is applied to examine the dynamics of an urbanbased language revitalization movement in the Autonomous Community of Galicia (North-western Spain). The potential of Resource Management Theory is explored as a way of systematically analysing the dynamics of urban-based language revitalization movements. It does this by identifying factors which both helped fuel the emergence and growth of this Galician grassroots movement as well as those constraining its potential development. Drawing on in-depth interviews and observations collected over six months of ethnographic fieldwork in one of Galicia’s main cities, social movement theory is used to analyse the role of Galician social movement activists as social agents in shaping the success of their language revitalization initiative. We argue that a social movement lens provides a useful analytical toolkit to focus on the grassroots efforts of social agents involved in peripheral ethnolinguistic mobilization in minority language contexts such as Galicia. Ultimately, we aim to show that these social movement revitalization initiatives go beyond language as an object and are centred around language-based struggles which not only address strategy dilemmas but also scaffold social relations and ties among speakers as they mobilize within particular institutional fields.

Traditional Atlantic Diet and Its Effect on Health and the Environment

The universal call to action for healthier and more sustainable dietary choices is the framework of the United Nations's Sustainable Development Goals. The Atlantic diet, originating from the northwest of the Iberian Peninsula, represents an example of a traditional diet that aligns with these principles. To explore a 6-month intervention based on the Atlantic diet's effects on metabolic and environmental health, assessing metabolic syndrome (MetS) incidence and the carbon footprint. The Galician Atlantic Diet study was a 6-month randomized clinical trial designed to assess the effects of this regional traditional diet on families' eating habits. The study was conducted from March 3, 2014, to May 29, 2015, at a local primary health care center in the rural town of A Estrada in northwestern Spain and involved a multisectoral collaboration. Families were randomly selected from National Health System records and randomized 1:1 to an intervention or control group. This secondary analysis of the trial findings was performed between March 24, 2021, and November 7, 2023. Over 6 months, families in the intervention group received educational sessions, cooking classes, written supporting material, and foods characteristic of the Atlantic diet, whereas those randomized to the control group continued with their habitual lifestyle. The main outcomes were MetS incidence, defined per National Cholesterol Education Program Adult Treatment Panel III guidelines, and carbon footprint emissions as an environmental metric using life cycle assessment with daily dietary intake as the functional unit. Initially, 250 families were randomized (574 participants; mean [SD] age, 46.8 [15.7] years; 231 males [40.2%] and 343 females [59.8%]). The intervention group included 126 families (287 participants) and the control group, 124 families (287 participants). Ultimately, 231 families completed the trial. The intervention significantly reduced the risk of incident cases of MetS (rate ratio, 0.32; 95% CI, 0.13-0.79) and had fewer MetS components (proportional odds ratio, 0.58; 95% CI, 0.42-0.82) compared with the control condition. The intervention group did not have a significantly reduced environmental impact in terms of carbon footprint emissions compared with the control group (-0.17 [95% CI, -0.46 to 0.12] kg CO2 equivalents/person/d). These findings provide important evidence that a family-focused dietary intervention based on a traditional diet can reduce the risk of incident MetS. Further research is needed to understand the underlying mechanisms and determine the generalizability to other populations, taking into account regional cultural and dietary variations. ClinicalTrials.gov Identifier: NCT02391701.

Basic Intonation Patterns of Galician Spanish

This paper presents an inventory of pitch accents and boundary tones in Galician Spanish (GS), a variety spoken in Northwestern Spain. Research so far has focused on explaining GS intonation features as transfer phenomena from Galician, the vernacular Romance language in the region. Because of this, previous studies have often included Galician L1 speakers, for whom transfer is expected when speaking Spanish L2. However, GS is the single L1 of half the children in Galicia, and it is spoken almost exclusively by about a quarter of Galicians. Our study focuses on this population and investigates the relative frequency and distribution of tonal units in GS when direct transfer from Galician is unlikely. A corpus of 1706 sentences (statements, questions, imperatives, and vocatives in neutral and biased contexts) was obtained from 28 participants through a discourse completion task. Results showed that patterns previously attributed to direct transfer from Galician L1 (for example, upstepped final accents in neutral declaratives or falling contours in unmarked interrogatives) are widespread in GS as L1. Findings also show commonalities with other L1 Spanish varieties, both in Europe (for example, L* L% as the unmarked declarative ending) and America (for example, the L* + H prenuclear accent of Caribbean varieties).

Exploring the influence of density-dependence and weather on the spatial and temporal variation in common vole (Microtus arvalis) abundance in Castilla y León, NW Spain.

The common vole has invaded the agroecosystems of northwestern Spain, where outbreaks cause important crop damage and management costs. Little is yet known about the factors causing or modulating vole fluctuations. Here, we used 11 years of vole abundance monitoring data in 40 sites to study density-dependence and weather influence on vole dynamics. Our objective was to identify the population dynamics structure and determine whether there is direct or delayed density-dependence. An evaluation of climatic variables followed, to determine whether they influenced vole population peaks. First- and second-order outbreak dynamics were detected at 7 and 33 study sites, respectively, together with second-order variability in periodicity (2-3 to 4-5-year cycles). Vole population growth was explained by previous year abundance (mainly numbers in summer and spring) at 21 of the sites (52.5%), by weather variables at 11 sites (27.5%; precipitation or temperature in six and five sites, respectively), and by a combination of previous abundance and weather variables in eight sites (20%). We detected variability in vole spatiotemporal abundance dynamics, which differs in cyclicity and period. We also found regional variation in the relative importance of previous abundances and weather as factors modulating vole fluctuations. Most vole populations were cyclical, with variable periodicity across the region. Our study is a first step towards the development of predictive modeling, by disclosing relevant factors that might trigger vole outbreaks. It improves decision-making processes within integrated management dealing with mitigation of the agricultural impacts caused by voles. © 2023 Society of Chemical Industry.

Asturian cider in Madrid? Linguistic identity and the multilingual signage of Asturian restaurants in Madrid, Spain

ABSTRACT Migrant communities settle and appropriate spaces in their new home through deterritorialisation and reterritorialisation processes, which involve the reconceptualisation of the language and symbols of the homeland and those spoken and used in the diaspora. The public sphere is one of the most distinguishable places where this contextual relation is expressed. This paper examines how language and semiotic resources are used in the linguistic landscape of Asturian restaurants in the capital of Spain, Madrid, to represent spaces this community had in their motherland, Asturias (North-western Spain). Following a multimodal analysis of signage in ten Asturian restaurants, it analyses sign-genres, the distinction between symbolic and communicative functions of the signage, the language choice in authenticating places, and the placement of signs in the semiotic landscape.

Applications of bioclimatology to assess effects of climate change on viticultural suitability in the DO León (Spain)

Spain accounts for 13.1% of the world’s vineyard area, and viticulture is crucial for the socioeconomic and cultural sectors. Vineyards are among the perennial crops that can suffer most negative impacts under climate change which can pose challenges to the sustainability of viticulture. Local and regional studies are needed to assess these impacts to help implement effective strategies in response to climate change. To this end, our approach involves integrating both conventional agroclimatic indices and those new bioclimatic indices that have proven to be essential for the characterization and demarcation of vineyards into species distribution models to assess areas suitable for viticulture under climate change projections. The proposed methodology was tested in a viticultural region located in northwestern Spain (DO León). An ensemble platform was used to build consensus models encompassing three general circulation models, two emission scenario pathways and two time horizons. Only the predictors that effectively characterize each grape variety were included in the models. The results revealed increases in the continentality index, compensated thermicity index, hydrothermic index of Branas, and temperature range during ripening in all the future scenarios analyzed in comparison to current conditions. Conversely, the values for the annual ombrothermic index and growing season precipitation may decrease in the future. The pattern of changes for 2070 will be more pronounced than for 2050. A significant loss of future habitat suitability was detected within the limits of the study area for the grape varieties analyzed. This negative impact could be counteracted to some degree with new and favorable areas for the cultivation of vineyards in territories located at the north of the DO limits. We suggest that our results could help policymakers to develop practices and strategies to conserve existing grape varieties and to implement efficient adaptation measures for mitigating or anticipating the effects of climate change on viticulture.

Modelling the impact land use change on flood risk: Umia (Spain) and Voglajna (Slovenia) case studies

Accurate quantification and assessment flood hazards is critical for mitigation and prevention. This study proposes a methodological framework for analysing this hazard and its relationship to agricultural and forestry land use. The objective is to obtain an integrative methodology based on the HEC-HMS model and to apply different simulations, with land use changes as indicator in two different study areas, taking into account the different characteristics of each basin and the different regulations of each area. This was done in two river basins, the Umia Basin (north-western Spain) and the Voglajna Basin (eastern Slovenia). The hydrological models obtained showed a very high performance in the calibration and validation periods. In the Umia River Basin agricultural use has priority over forestry, with food security taking precedence over water security. Reforestation only upstream has been shown to be almost as beneficial as reforestation throughout the basin. However, the use of abandoned land currently limits the reduction of peak discharge. Nevertheless, for an event designed with a 100-years return period, a reduction of about 12% was achieved. The increase in agricultural use promoted by the administration could increase this peak by about 6%, thus increasing the flood hazard, but it has been shown that this use on less permeable soils and upstream would not significantly increase this peak (<1% for the event studied). On the other hand, the Voglajna Basin has a smaller catchment area and fragmented land use, and a mosaic landscape. For the 100-years return period the reduction in peak discharge is only few percent compared to the baseline scenario even if 30% of the agricultural land (about 7% of the total area) is changed to forest land use. The information provided by the simulations is a useful indicator that can be incorporated into management plans to ensure appropriate decision making by the administration. Not only for the application of nature-based solutions (NBS) and providing evidence-base for the NBS and the reduction of flood risk, but such information is key when the use changes from forestry to agriculture and vice versa, as it provides tools to ensure food and water security. In both case studies it was demonstrated how stakeholders need to undertake optimal and strategic planning and management in order to reduce the risk of flooding. In turn, the use of this modelling, as well as the calculation of scenarios from a perspective that not only evaluates different land use changes, but also incorporates different regulations, is presented as an innovative and realistic analysis.

Relationship between two pathogens in an amphibian community that experienced mass mortalities.

Because host species tend to harbor multiple parasitic species, coinfection in a host is common. The chytrid fungus Batrachochytrium dendrobatidis (Bd) and the viruses in the genus Ranavirus (Rv) are responsible for the decline of amphibians worldwide. Despite wide geographical co-occurrence and the serious conservation problem that coinfection with these pathogens could represent, little is known about their possible synergistic interactions and effects in a host community. We investigated the occurrence and associations between these two pathogens in an amphibian community after Rv-driven disease outbreaks were detected in four populations of the Iberian ribbed newt (Pleurodeles waltl) in northwestern Spain. We collected tissue samples from amphibians and fish and estimated Bd and Rv infection loads by qPCR. A few months after the most recent mass mortality event, Rv infection parameters at the affected sites decreased significantly or were lower than such registered at the sites where no outbreaks were recorded. Both pathogens were simultaneously present in almost all sites, but coinfection in a single host was rare. Our findings suggest that the co-occurrence of Bd and Rv does not predict adverse outcomes (e.g., enhanced susceptibility of hosts to one pathogen due to the presence or infection intensity of the other) following an outbreak. Other variables (such as species identity or site) were more important than infection with a pathogen in predicting the infection status and severity of infection with the other pathogen. Our results highlight the importance of host-specific and environmental characteristics in the dynamics of infections, coinfection patterns, and their impacts.

Temporal trends of land-use favourability for the strongly declining little bustard: assessing the role of protected areas.

The little bustard (Tetrax tetrax) is a steppe bird strongly and negatively influenced by agricultural intensification in Europe. Here, we use the little bustard as a model species to examine how favourability (relative occurrence likelihood of a species based on environmental characteristics, such as habitat availability) varies regionally with degree of protection in north-western Spain. The Natura2000 network is one of the main biodiversity conservation tools of the European Union, aiming to protect areas hosting species of conservation concern from unfavourable land-use changes. The network covers many landscapes across the continent, including farmland. Additionally, we examine the relationship between trends in land-use favourability and little bustard population trends over a decade in the Nature Reserve of Lagunas de Villafáfila, a protected area also in the Natura2000 network where active and intense management focused on steppe bird conservation is carried out. Favourability was much greater in Villafáfila than in both protected areas with lower degree of protection and in non-protected areas. Land-use favourability increased slightly between 2011 and 2020 both in and out of protected areas, whereas little bustard populations declined sharply in that period, even in Villafáfila. Spatial variations in little bustard abundance within Villafáfila depended on social attraction (increasing with the number of neighbouring males) but not significantly on small-scale variations in land-use favourability. These results suggest that land-use management in Natura2000 areas needs to be more conservation-focused, favouring natural and seminatural habitats and traditional farming practices to improve land-use favourability for little bustards and other steppe birds. Additional factors, such as field-level agricultural management or social interaction variables that may cause an Allee effect, should be incorporated in little bustard favourability models to improve their use in conservation planning.

Updating catastrophe models to today’s climate – An application of a large ensemble approach to extreme rainfall

As climate change unfolds, extreme weather events and natural hazards relevant for the insurance industry may change in frequency and intensity. In order to guarantee risk-adequate pricing, risk modelers have to ask themselves the question whether a certain ‘hazard’ parameter based on past observations and calibrated on past data is still valid or needs to be updated. Using the example of heavy rainfall between 3 h and 72 h as a proxy for flash flooding, we apply a unique high-resolution single model initial-condition large ensemble. We outline a methodology by which risk modelers can assess whether, where and by how much updating the hazard component of their risk model is needed. Therefore, we compare two time periods: 1980–1999 (as an example for a typical baseline period) and 2015–2034 (representing a 20-year period centered around today). We argue that assessing changes over the full ensemble space is vital for (i) the identification of homogeneous regions where a certain signal emerges, and (ii) the quantification of risk changes in the tail of the extreme value distribution that may still be hidden in the mean response to climate change. In the example case of 3-hourly 50-year rainfall return levels, we find a significant increase between 1980 and 1999 and 2015–2034 over 44 % of European land area. We also identify specific risk regions such as northwestern Spain where changes in the very tail (100-year rainfall return level) already emerge before more common extremes (1-year rainfall return level). Failing to detect and consider these tail changes or assuming equal changes for common and rare extremes may therefore lead to an under- or overestimation of the true level of risk today.

Mycena fragosa (Mycenaceae), a new species from the fragas in northwestern Spain

A new species of Mycena from northwestern Spain is described, illustrated and morphologically and molecularly compared to related species. The new species belongs to M. sect. Viscipelles, presenting as tiny in stature, with small spores, claviform to subglobose cheilocystidia with short excrescences, and a small disc at the base of the stipe, which is made up of long cystidia. Currently, it has been only found at three localities of Atlantic forest, where it grows on dead leaves of Alnus lusitanica, Acer pseudoplatanus, Salix atrocinerea, and Quercus robur acorn cupules. In addition, a key to the taxa of M. sect. Viscipelles, which are known in the Northern Hemisphere, is provided

Hiding from heat: The transcriptomic response of two clam species is modulated by behaviour and habitat

Rising occurrence of extreme warming events are profoundly impacting ecosystems, altering their functioning and services with significant socio-economic consequences. Particularly susceptible to heatwaves are intertidal shellfish beds, located in estuarine areas already stressed by factors such as rainfall events, red tides, eutrophication, and pollution. In Galicia, Northwestern Spain, these beds support vital shellfisheries, featuring the native clam Ruditapes decussatus and the non-indigenous R. philippinarum. Over recent decades, these populations have experienced notable abundance shifts due to various anthropogenic impacts, including climate change. In this habitat, patches of the seagrass Zostera noltei that coexist with bare sand can act as thermal refuges for benthic organisms such as clams. To assess the impact of heatwaves on these ecosystems, a mesocosm experiment was conducted. Juveniles of both clam species in two habitat types—bare sand and sand with Z. noltei—were exposed to simulated atmospheric heatwaves during diurnal low tide for four consecutive days. Subsequent transcriptomic analysis revealed that high temperatures had a more pronounced impact on the transcriptome of R. philippinarum compared to R. decussatus. The habitat type played a crucial role in mitigating heat stress in R. philippinarum, with the presence of Z. noltei notably ameliorating the transcriptomic response. These findings have direct applications in shellfishery management, emphasizing the importance of preserving undisturbed patches of Z. noltei as thermal refuges, contributing to the mitigation of heatwave effects on shellfish populations.

The Southern European Atlantic diet and all-cause and cause-specific mortality: a European multicohort study.

The Southern European Atlantic diet (SEAD) is the traditional dietary pattern of northwestern Spain and northern Portugal, but it may resemble that of central, eastern, and western European countries. The SEAD has been found associated with lower risk of myocardial infarction and mortality in older adults, but it is uncertain whether this association also exists in other European populations and if it is similar as that found in its countries of origin. We conducted a prospective analysis of four cohorts with 35 917 subjects aged 18-96 years: ENRICA (Spain), HAPIEE (Czechia and Poland), and Whitehall II (United Kingdom). The SEAD comprised fresh fish, cod, red meat and pork products, dairy, legumes and vegetables, vegetable soup, potatoes, whole-grain bread, and moderate wine consumption. Associations were adjusted for sociodemographic variables, energy intake, lifestyle, and morbidity. After a median follow-up of 13.6 years (range = 0-15), we recorded 4 973 all-cause, 1 581 cardiovascular, and 1 814 cancer deaths. Higher adherence to the SEAD was associated with lower mortality in the pooled sample. Fully adjusted hazard ratios and 95% confidence interval per 1-standard deviation increment in the SEAD were 0.92 (0.89, 0.95), 0.91 (0.86, 0.96), and 0.94 (0.89, 0.99) for all-cause, cardiovascular, and cancer mortality, respectively. The association of the SEAD with all-cause mortality was not significantly different between countries [Spain = 0.93 (0.88, 0.99), Czechia = 0.94 (0.89,0.99), Poland = 0.89 (0.85, 0.93), United Kingdom = 0.98 (0.89, 1.07); P for interaction = 0.16]. The SEAD was associated with lower all-cause, cardiovascular, and cancer mortality in southern, central, eastern, and western European populations. Associations were of similar magnitude as those found for existing healthy dietary patterns.

Detection of different Betanodavirus genotypes in wild fish from Spanish Atlantic coastal waters (Galicia, northwestern Spain).

The nervous necrosis virus (NNV; genus Betanodavirus) is an aquatic pathogen that is responsible for a neurological disease affecting marine fish. Despite its almost worldwide distribution, global warming could favor the spread of NNV to new areas, highlighting the importance of conducting epidemiological surveys on both wild and farmed marine fish species. In this study, we assessed NNV prevalence in wild fish caught along the Galician Atlantic coast. In total, 1277 fish were analyzed by reverse transcription real-time polymerase chain reaction. Twenty two (1.72%) of those fish tested positive for NNV, including two species in which the pathogen had not yet been reported. The reassortant RGNNV/SJNNV (red-spotted grouper NNV/striped jack NNV) was detected in 55% of NNV-positive individuals, while the remaining 45% harbored the SJNNV-type genome. Moreover, from European Pilchard Sardina pilchardus and Atlantic Mackerel Scomber scombrus, we isolated four reassortant strains that carried amino acid mutations at key sites related to NNV-host interaction.